Identification of Campylobacter Jejuni and Campylobacter Coli from Diarrheic Samples Using PCR

Authors

  • Babak Negahdari 1 1. Dept. of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Mohamad Hasan Shirazi 2 2. Dept. of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran.
  • Ziba Vaise Malekshahi 1
  • Maryam kadkhodazadeh 3 3. Dept. of Clinical Biochemistry, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
  • Sara Hajikhani 2
  • Majid Rahmati 4* 4. Dept. of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

DOI:

https://doi.org/10.22100/ijhs.v2i2.83

Keywords:

Campylobacter jejuni, Campylobacter coli, Isolation, PCR.

Abstract

Background: Campylobacter jejune and C. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. In this study Identification of Campylobacter Jejuni and Campylobacter Coli from samples using PCR was explored.

Methods: Detection was performed using diarrheal samples collected 8 from 117 children. The genomic DNA of samples was extracted by phenol-chloroform method. All DNA extracts were examined for the presence of C.jejuni and C.coli species based on PCR method.

Results: Of 117 diarrheal samples, 35 (29.9%) were found positive for 10 Campylobacter spp using PCR.

Conclusions: The results of this study showed that PCR is effective for rapidly screening stool samples for Campylobacter spp, due to its high sensitivity and specificity.

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Published

2016-06-12

Issue

Volume 2, Issue 2, 2016

Section

Articles

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How to Cite

Identification of Campylobacter Jejuni and Campylobacter Coli from Diarrheic Samples Using PCR. (2016). International Journal of Health Studies, 2(2). https://doi.org/10.22100/ijhs.v2i2.83