The Effect of Eight Weeks Aerobic Training with Citrus Aurantium on miR-1 and miR-22 in Left Ventricle of Elderly Female Rats; An Experimental Study
Authors
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Anahita Sharifi
Department of Physical Education & Sport Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
https://orcid.org/0000-0002-7373-879X
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Farshad Ghazalian
Department of Physical Education and Sport Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
https://orcid.org/0000-0002-7315-1382
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Hossein Abednatanzi
Department of Physical Education & Sport Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
https://orcid.org/0000-0001-6638-1131
DOI:
https://doi.org/10.22100/ijhs.v10i4.1148Keywords:
Training, Citrus Aurantium, miR-1, miR-22, Left ventricle, ElderlyAbstract
Background: Heart attack is the main cause of mortality in the elderly. Although the beneficial role of exercise and medicinal plants on heart health has been reported, the simultaneous effect of these factors on micromolecular pathways in heart tissue is still unknown. This study aimed to investigate the effect of eight weeks aerobic training (AT) with Citrus Aurantium (CA) on miR-1 and miR-22 in left ventricle of elderly female rats.
Methods: In this experimental study, 35 female Sprague-Dawley rats (age 14-18 months and weight of 270-320 g) were randomly divided into (1) control (C), (2) sham (normal saline/Sh) (3) CA, (4) AT and (5) AT+CA groups. The AT groups performed aerobic training for eight weeks, three sessions per week each lasting 25-41 minutes at a speed of 20-27 meters per minute. The CA groups received 300 mg/kg CA intra-peritoneally. One-way analysis of variance with Tukey's post-hoc tests was used for statistical data analysis (Pvalue≤0.05).
Results: miR-1 and miR-22 levels in AT and AT+CA groups were significantly higher than in C and CA groups (P-value=0.001). miR-1 levels in AT+CA group were significantly lower than AT group (P-value=0.005) and miR-22 levels in AT group were significantly lower than AT+CA group (P-value=0.001).
Conclusions: Aerobic training seems to be an effective factor on the improvement of miRs involved in left ventricular hypertrophy; nevertheless, Citrus Aurantium has no significant effect, or even modulates the effect of training.
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