Effect of Different Activation Mechanism of T Cells on Immunoregulatory Properties of Bone Marrow Mesenchymal Stem Cells

Authors

  • Masoomeh Masoomikarimi 1 1 School of Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
  • Raziyeh Alipour 2 2 Dept. of Immunology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
  • Minu Adib 2
  • Reza Ahmadi 3 3 Biochemistry Center, Sahrekord University of Medical Science, Sahrekord, Iran.
  • Moslem Jafarisani 4* 4 School of Medicine, Shahroud University of Medical Science, Shahroud, Iran.
  • Agheel Tabar Mollahassan 5 5 Dept. of Immunology, School of Medical Science, Islamic Azad University Babol Branch, Babol, Iran.

DOI:

https://doi.org/10.22100/ijhs.v1i3.74

Keywords:

Mesenchymal stem cell, T cells, Immunoregulatory, TGF-b, IDO

Abstract

Background: Mesenchymal Stem Cells (MSC) have ability to regulate immune response via proinflamatory cytokines. In this study we aimed to investigate the effect of different mechanism for T cell activation and adjacency on immunoregulatory properties of MSC.

Methods: Bone marrow MSCs were purchased and T cells were obtained from whole blood sample. T cells were activated by two different mechanisms using Peripheral Blood Mononuclear Cell (PBMC) and phytohemagglutinin (PHA) and then co-cultured with MSC using transwell cultures (Indirect contact) and usual plate (Direct contact). After 96 hours TGF-b concentration in culture medium and indole amine 2, 3-dioxygenase (IDO) activity in cell lysate were assayed. We used ANOVA and T-test for statistical analysis and 0.05 was considered as significant level.

Results: Our result showed a significant increase of TGF-b secretion and IDO activity. Increase in mixed lymphocyte culture (MLC) groups was more significant than lymphocyte transformation test (LTT) in compare with control.

Conclusions: The present study confirmed immunoregulatory effect of BM-MSC. Also this study showed that MLC of BM-MSCs and T cells have more immunoregulatory effects than LTT.

 

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Published

2016-01-26

Issue

Volume 1, Issue 3, 2015

Section

Articles

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How to Cite

Effect of Different Activation Mechanism of T Cells on Immunoregulatory Properties of Bone Marrow Mesenchymal Stem Cells. (2016). International Journal of Health Studies (Undergoing Change to Shahroud Journal of Medical Sciences), 1(3), page:35-39. https://doi.org/10.22100/ijhs.v1i3.74