Protective Effects of Hydroxytyrosol on Renal Ischemia-reperfusion Injury in Mice

  • Nooshin Ahmadian Chashmi1 1. Vice-Chancellery of Health, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Sarvenaz Emadi 2 2. School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
  • Hossein Khastar 3* 3. Dept. of Physiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Abstract

Background: Renal ischemia reperfusion (IR), one of the most important causative mechanisms of acute kidney injury, is a major clinical problem that occurs in some hospitalized patients. The aim of this study was to investigate the effects of hydroxytyrosol (HT) on renal ischemia-reperfusion injury in mice.Methods: Male mice were randomly assigned to three groups (N = 9): (1) sham operated, (2) renal IR (45 min ischemia followed by 24 h reperfusion), or (3) renal IR+HT (5 mg/kg orally for 7 days before renal IR and one hour before IR). Animals were sacrificed and the blood and kidney tissue samples were collected for glutathione (GSH) and malondialdehyde (MDA) evaluation.Results: Renal IR injury led to decreases in renal tissue GSH and increases in MDA levels compared to sham operated. Hydroxytyrosol partially inhibited the IR-induced decrease in GSH activity. In addition, in the renal IR+HT group, MDA was increased compared to the renal IR group.Conclusions: These findings suggest that HT diminished oxidative stress in renal tissue after renal IR.
Published
Nov 16, 2016
How to Cite
AHMADIAN CHASHMI1, Nooshin; EMADI 2, Sarvenaz; KHASTAR 3*, Hossein. Protective Effects of Hydroxytyrosol on Renal Ischemia-reperfusion Injury in Mice. International Journal of Health Studies, [S.l.], nov. 2016. ISSN 2423-6594. Available at: <http://ijhs.shmu.ac.ir/index.php/ijhs/article/view/148>. Date accessed: 06 dec. 2019. doi: http://dx.doi.org/10.22100/ijhs.v2i3.148.
Section
Articles

Keywords

Ischemia reperfusion, Acute kidney injury, Hydroxytyrosol, Oxidative stress.