Brucella Antibody Levels in Preschool Children in the North East of Iran
Background: Brucellosis is a zoonotic disease of humans and animals
that has affected worldwide. Iran is one of the endemic areas infected
with brucellosis. Early diagnosis of this disease may protect the
affected children from disabilities and mortalities. This study aimed to
evaluate the Brucella antibody levels in preschool children of the
Shahroud city in Iran.
Methods: This cross-sectional study evaluated 850 participants, in
particular, school children from the Shahroud city. General
information was collected by interviewing the children’s parents.
Moreover, a 5 ml of blood sample was collected from all the children.
The samples were studies using standard tube agglutination test
(Wright) and 2-Mercaptoethanol. Thereafter, IgG and IgM levels were
determined by the ELISA method.
Results: A total of 850 children were enrolled, of which 51.2% were
boys and 48.8% were girls, with a mean age of 5.17±1.55 years. Of the
all children evaluated, 839 (97.5%) children had a titer <1/80 and 21
children (2.5%) had a titer ≥1/80. A significant difference was
observed between the antibody titers in terms of gender (P=0.012),
whereas no significant association was found among antibody titers
with other variables such as age, history of nonpasteurized foods,
exposure to animals, history of brucellosis disease, and parental
Conclusions: The antibody titer for suspected brucellosis in preschool
children of Shahroud was very low. According to the results of our
study, in particular, in the endemic areas, a Wright’s titer of 1/80 in
suspected cases for brucellosis can be considered as a diagnostic titer.
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