Poly-lactic Acid/Gelatin Nanofiber (PLA/GTNF) Conduits Containing Platelet-Rich Plasma for Peripheral Nerve Regeneration

  • Saeed Farzamfar1 1. Dept. of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Farshid Esmailpour1
  • Majid Rahmati2 2. Dept.of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Ahmad Vaez3 3. Dept. of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Mehdi Mirzaii4 4. Dept. of Microbiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Behzad Garmabi2
  • Asghar Shayannia2
  • Elham Ebrahimi5 5. Dept. of Midwifery, Maragheh Branch, Islamic Azad University, Maragheh Iran.
  • Hamid Vahedi6* 6. Clinical Research Development Unit, Imam Hossein Hospital, Shahroud University of Medical Sciences, Shahroud, Iran.
  • Majid Salehi7** 7. Dept. of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Abstract

Background: Polymeric scaffolds have achieved immense importance in the field of nerve tissue engineering.Methods: In the present study, the combination of thermally induced phase separation (TIPS) and electrospinning methods were used to fabricate poly- (lactic acid)/gelatin nanofiber/PRP-scaffolds. Several physical and mechanical tests (weight loss measurement, surface wettability, porosity, microstructure observation via SEM photography, mechanical tests such as tensile strength, and Young modulus) and cellular assays (MTT assay and DAPI staining) were explored to assess the scaffolds capability to serve as neural guidance conduit. In this study, we hypothesized that conduits enriched with PRP may provide a better regenerative environment for nerve tissue repair. Results: This study suggests that GTNF/PRP incorporated scaffolds revealed better biological and physical properties than PLA only scaffolds.Conclusions: Results indicate that when GTNF/PRP is incorporated into the PLA scaffolds, resultant mechanical properties, porosity and cell attachment, and viability in vitro were better than pure PLA.
Published
Sep 18, 2017
How to Cite
FARZAMFAR1, Saeed et al. Poly-lactic Acid/Gelatin Nanofiber (PLA/GTNF) Conduits Containing Platelet-Rich Plasma for Peripheral Nerve Regeneration. International Journal of Health Studies, [S.l.], sep. 2017. ISSN 2423-6594. Available at: <http://ijhs.shmu.ac.ir/index.php/ijhs/article/view/236>. Date accessed: 15 dec. 2019. doi: http://dx.doi.org/10.22100/ijhs.v3i2.236.
Section
Articles

Keywords

Thermally induced phase separation (TIPS), Poly-lactic acid/gelatin Nanofiber (PLA/GTNF), Platelet rich plasma (PRP).