The Acute Inflammatory Response Induced by Fe2Nio4 Nanoparticles: In Vivo Evaluation
AbstractBackground: Despite the ever-increasing beneficial applications of magnetic nanoparticles in biological systems, few studies have been carried out on the combined effects of nanoparticles (Fe and Ni) on human health. In the present study the effects of Fe2NiO4 nanoparticle on inflammatory factors were assessed. Methods: Twenty-four Wistar rats were randomly divided into 3 groups: two groups were treated with Fe2NiO4 nanoparticle; (2,3) and the third group was taken as the control group (1). Group 2 and 3 received 0.5ml of solution containing 100 and 200 ppm Fe2NiO4 for 7 successive days, respectively. Inflammatory cytokines such as IL-6 and Tumor necrosis factorα (TNFα) were measured at various time points (1, 2, 7 and 14 days) by ELISA kits. After 14 days, lung tissue was investigated. Results: On day 7, IL-6 concentration significantly increased in-group2. Also during the 14 days, IL-6 concentration significantly increased in groups1and2 in comparison with control group. The concentration of TNFα on 7th day significantly increased in groups 2 and 3 and on day 14th, TNFα concentration significantly increased in groups 2 and 3compared to control group. There were no pathological changes between the control group and group 2 but in-group3, signals of fibrous and thickening of the air sacs were observed. Conclusions: In the present study we found that inflammatory cytokines (IL-6 and TNFα) increased in the groups treated with Fe2NiO4 and high dose of this nanoparticles caused drastic changes in lung tissue.
The Copyright Form should be downloaded and signed by corresponding author in the fourth step "upload supplementary files" during submission process.
After acceptance, copyright form should be downloaded and signed by all authors one by one ( "summery --> supp. file" part and click on "add a supplementary file" link).