Effect of Thymus Vulgaris Ethanol Extract, on Serum Total Antioxidant in Experimental Induced Poly Cystic Ovarian Syndrome (PCOs) Rats
Background: Poly cystic ovary syndrome is one of the most common female endocrine disorders. One of the side effects of PCOS is oxidative stress.. Here we investigated antioxidants effects of Thymus vulgaris ethanoli extract on experimental PCOS induced rats by estradiol-valerat (PPA).
Methods: Wistar female rat (n=70) were divided into 7 groups including C1: an equal volume of (0.9% NaCl) as placebo; C2: extract (0.6cc/rat/orally/daily); C3: induced PCO by single injection of estradiol-valerate (4mg/rat/IM), and T1: PCOS induced rats + an equal volume of (0.9% NaCl) as placebo, T2: PCOS induced rats + extract(0.2cc/rat/orally/daily), T3: PCOS induced rats + extract (0.4cc/rat/orally/daily), T4:PCOS induced rats+extract (0.4cc/rat/orally/daily) test groups, were received extract supplement, for 60 consequence days. Animals were kept in standard conditions. In last day of study the blood samples of rats in whole groups were obtained and prepared to biochemical analysis.
Results: Total antioxidant capacity level, superoxide dismutase and catalase activity were significantly increased in PCOS treated groups (P<0.03), these parameters in PCOS groups that did not receive extract significantly decreased (P<0.05) in comparison to control. Level of MDA in PCOS groups were significantly increased as compared to control and extract treated groups (P<0.01).
Conclusions: Our results disclosed that administration of Thymus vulgaris ethanol extract significantly restitution tissue antioxidants level in PCOS induced rats.